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Σημαντικές πληροφορίες σχετικές με τη μικροσκοπία TIRFM
Αρχή της TIRFM
Επιπλέον γνώση σχετικά με το δείκτη διάθλασης
https://en.wikipedia.org/wiki/Refractive_index και το παρακάτω
Total Internal Reflection Fluorescence Microscopy (TIRFM) is a type of microscopy that allows for high-resolution imaging of fluorescent molecules located near a surface. TIRFM is based on the principle of total internal reflection, which occurs when light travels from a medium of higher refractive index to a medium of lower refractive index at an angle greater than the critical angle.
In TIRFM, a laser beam is directed through a high-refractive index material such as a glass prism, at an angle greater than the critical angle. This creates an evanescent wave that penetrates the sample to a depth of approximately 100 nanometers. Fluorescent molecules within this range are excited by the evanescent wave and emit fluorescence, which can be detected by a sensitive camera.
TIRFM is particularly useful for studying cellular processes that occur at or near the cell membrane, such as receptor-ligand interactions, vesicle trafficking, and cell adhesion. By selectively illuminating the molecules located near the surface of the cell, TIRFM can provide high-resolution images with minimal background fluorescence.
TIRFM has a wide range of applications in biological research, including the study of cell biology, neurobiology, and developmental biology. It has the potential to provide insights into the mechanisms that govern cellular function and disease, and has been used to study a variety of biological processes, such as signaling, membrane trafficking, and cell migration.
Ένα άρθρο ανασκόπησης
Total Internal Reflection Fluorescence (TIRF) Microscopy
MOSCHOU LAB 